Welcome

Opening lecture and introduction to wet lab practices and master-classes

Wet lab practices on single cell sequencing (full day):

• Sample preparation: solutions from RWD Life Science
• SeekGene  single cell full-length RNA library preparation
• SeekGene  5' single cell RNA library preparation

Satellite workshop on digital PCR (~ 2 hours):

• Master-class on Sample preparation using the GenePure Pro automatic nucleic acid isolation system
• Master-class on Sniper DQ24 digital PCR
  

• Master-class on Maccura D600 digital PCR
  

Hands-on advanced bioinformatics:

Moderators: Pavel Yamshikov and Anna Khozyainova

• scRNA-seq and spatial transcriptomics bioinformatics
• Gene regulatory networks
• Analysis of multimodal single cell omics
• Somatic variation discovery
• Analysis of dynamics processes
• Analysis of transcribed cis-regulatory elements
• Cell communication
• AI in data analysis

Wet lab practices on single cell sequencing (full day, continuation):

• Sample preparation: solutions from RWD Life Science
• SeekGene single cell full-length RNA library preparation
• SeekGene  5' single cell RNA library preparation

Satellite workshop on digital PCR (~ 2 hours, continuation):

• Master-class on Sample preparation using the GenePure Pro automatic nucleic acid isolation system
• Master-class on Sniper DQ24 digital PCR
  

• Master-class on Maccura D600 digital PCR
  

Hands-on advanced bioinformatics (continuation)

Master-class on LongCyte flow cytometry

Satellite workshop on digital PCR (~ 2 hours):

• Master-class on Sample preparation using the GenePure Pro automatic nucleic acid isolation system
  

• Master-class on Sniper DQ24 digital PCR
• Master-class on Maccura D600 digital PCR
  

Hands-on basic bioinformatics:

Moderators: Pavel Yamshikov and Anna Khozyainova

• Server Work
• Basic bioinformatics of bulk RNA-seq
• Introduction to scRNA-seq and spatial transcriptomics bioinformatics
• Sample demultiplexing, barcode processing, gene counting
• QC, normalization methods, dimensional reduction, clustering approaches, UMAP/tSNE, differential expression
• Cell typing
• Sample integration and batch effect correction
• AI in data analysis

Hackathon on advanced bioinformatics:

Moderators: Marat Sabirov and Anna Valyaeva

• focus on single cell seq, spatial transcriptomics, multiomics and machine learning
• 4 biological problems
• 4 teams of 5 people each
• providing server resources
• presenting talks on the round table (Day 5, August 30)
• 2 prizes

Master-class on GeneMind Genolab M sequencing

Master-class on Third-generation sequencing

Satellite workshop on digital PCR (~ 2 hours, continuation):

• Master-class on Sample preparation using the GenePure Pro automatic nucleic acid isolation system
• Master-class on Sniper DQ24 digital PCR
• Master-class on Maccura D600 digital PCR
  

Hands-on basic bioinformatics (continuation)

Hackathon on advanced bioinformatics (continuation)

The round table “Experience in Single-Cell Analysis”:

• Talks by leading researchers

• Presentations from bioinformatics hackathon teams 

• Partner presentations and exhibition

Party